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. 2018 Sep 24;62(10):e00758-18. doi: 10.1128/AAC.00758-18

TABLE 3.

Strains and plasmids used in this study

Strain or plasmid Description Reference or source
Strains
    E. faecalis
        OG1RF Human oral cavity isolate 40, 41
        OG1RF ΔPIP PIP deletion strain 15
        OG1RF-C OG1RF ΔPIP ϕNPV1-resistant strain This work
        OG1RF ΔbgsB OG1RF bgsB deletion mutant This work
        OG1RF ΔepaR OG1RF epaR deletion mutant This work
        OG1RF ΔbgsB ΔepaR OG1RF bgsB and epaR double-deletion mutant This work
        OG1RF ΔepaR_Ew OG1RF ΔepaR with wild-type epaR complementation in cis This work
        OG1RF ΔepaR_Ec OG1RF ΔepaR complemented with OG1RF-C epaR allele in cis This work
        OG1RF ΔbgsB_Bw OG1RF ΔbgsB with wild-type bgsB complementation in cis This work
        OG1RF ΔbgsB_Bc OG1RF ΔbgsB complemented with OG1RF-C bgsB allele in cis This work
        OG1RF_R2 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R3 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R4 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R5 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R6 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R7 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R8 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R9 OG1RF ϕNPV1-resistant strain This work
        OG1RF_R10 OG1RF ϕNPV1-resistant strain This work
    E. coli
        EC1000 Cloning host, provides repA in trans 42
Plasmids
    pLT06 Cloning vector, temp-sensitive repA, Cmra 36
    pLT06_ΔbgsB bgsB deletion construct This work
    pLT06_ΔepaR epaR deletion construct This work
    pLT06_Ew epaR wild-type allele complementation construct This work
    pLT06_Ec epaR OG1RF-C allele complementation construct This work
    pLT06_Bw bgsB wild-type allele complementation construct This work
    pLT06_Bc bgsB OG1RF-C allele complementation construct This work
a

Cmr, chloramphenicol resistant.