TABLE 3.
Strains and plasmids used in this study
Strain or plasmid | Description | Reference or source |
---|---|---|
Strains | ||
E. faecalis | ||
OG1RF | Human oral cavity isolate | 40, 41 |
OG1RF ΔPIP | PIP deletion strain | 15 |
OG1RF-C | OG1RF ΔPIP ϕNPV1-resistant strain | This work |
OG1RF ΔbgsB | OG1RF bgsB deletion mutant | This work |
OG1RF ΔepaR | OG1RF epaR deletion mutant | This work |
OG1RF ΔbgsB ΔepaR | OG1RF bgsB and epaR double-deletion mutant | This work |
OG1RF ΔepaR_Ew | OG1RF ΔepaR with wild-type epaR complementation in cis | This work |
OG1RF ΔepaR_Ec | OG1RF ΔepaR complemented with OG1RF-C epaR allele in cis | This work |
OG1RF ΔbgsB_Bw | OG1RF ΔbgsB with wild-type bgsB complementation in cis | This work |
OG1RF ΔbgsB_Bc | OG1RF ΔbgsB complemented with OG1RF-C bgsB allele in cis | This work |
OG1RF_R2 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R3 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R4 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R5 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R6 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R7 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R8 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R9 | OG1RF ϕNPV1-resistant strain | This work |
OG1RF_R10 | OG1RF ϕNPV1-resistant strain | This work |
E. coli | ||
EC1000 | Cloning host, provides repA in trans | 42 |
Plasmids | ||
pLT06 | Cloning vector, temp-sensitive repA, Cmra | 36 |
pLT06_ΔbgsB | bgsB deletion construct | This work |
pLT06_ΔepaR | epaR deletion construct | This work |
pLT06_Ew | epaR wild-type allele complementation construct | This work |
pLT06_Ec | epaR OG1RF-C allele complementation construct | This work |
pLT06_Bw | bgsB wild-type allele complementation construct | This work |
pLT06_Bc | bgsB OG1RF-C allele complementation construct | This work |
Cmr, chloramphenicol resistant.