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Recovery of V. cholerae rod morphology on agarose pads. (A) Sphere anatomy after 3 h of treatment with PenG. OM, outer membrane; IM, inner membrane; C, cytoplasm; P, periplasm. Cellular compartments were determined as described in reference 5 using fluorescent protein fusions with known localization patterns. Scale bar, 1 μm. (B) Representative time-lapse images of PenG-generated spheres after removal of the antibiotic on an agarose pad.
