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Sphere recovery in liquid medium. Cells were grown to a density of ∼2 × 108 CFU/ml (T0) in minimal medium, exposed to penicillin G (100 μg ml−1, 10× MIC) for 3 h (T3), washed twice to remove the antibiotic, and then imaged every hour. Yellow arrowheads show rod-shaped cells, and the red arrowhead (plus enlarged window) shows sphere apparently budding of a rod.
