FIG 6.

An LPS core mutant is defective in sphere recovery and organization. (A) Time-dependent viability experiment in the presence of PenG. Strains were exposed to PenG (100 μg ml⁻¹, 10× MIC) at T0 and plated for CFU ml⁻¹ at the indicated times. The vc0225 mutant exhibits a significant plating defect by T3 (****, P < 0.0001). (B) Localization of PBP1a in PenG-treated wt (top) and mutant spheres (bottom). PBP1amCherry was expressed from its native, chromosomal locus. Scale bar, 5 μm. (C) Recovery pattern in a PenG-treated PBP1amCherry vc0225 mutant after exposure to and subsequent removal of PenG. Frames are 10 min apart.