TABLE 3.
Primers used in this study
| Primer name | Primer sequence (5′–3′)a |
|---|---|
| CgCDR1A | CATAGATCAGGGCCCATTACATTAGCACAG |
| CgCDR1B | CTCAGTGTTGCTCGAGATAGGGTTGATAC |
| CgCDR1C | GTTCTGTTAGTTCCGCGGACTCTCGTAGAT |
| CgCDR1D | GTGAATACAAACAAGAGCTCCACAATAATA |
| CgPDH1A | AAACAGTCTATGGTACCACAAGTTTGCACA |
| CgPDH1B | CCGTATACGTTTCGGGCCCTTGTCATCAA |
| CgPDH1C | ACAGAAGATGCGGCCGCTATGGTATATTTATT |
| CgPDH1D | ATTCCTTAATAACCGCGGAAGTTGACTTTA |
| CgSNQ2A | TTGAGTATCTTAGGGCCCTTGTTTTCAGTT |
| CgSNQ2B | GATAGAATACTCGAGTTGTCGCTGTGCGC |
| CgSNQ2C | GCTATTTATTACCGCGGCCATGTCAGAG |
| CgSNQ2D | AGACAGATATTGAGCTCCACTACTGCTGAG |
a
Underlined bases indicate the introduction of restriction enzyme cloning sites to allow directional cloning into the SAT1-flipper cassette.