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. 2017 Aug 29;38(5):2053–2060. doi: 10.1007/s10792-017-0697-x

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Effects of berberine on MCP-1 mRNA and IL-8 mRNA expression after stimulation with LPS in ARPE-19 cells in serum-free media were preincubated for 30 min with berberine or 0.05% DMSO. LPS (1 μg/ml) was added to the media and incubated for 3 h. After incubation, total RNA was extracted from the ARPE-19 cells, and semiquantitative real-time PCR was performed. Changes in MCP-1 mRNA (a) and IL-8 mRNA (b) in ARPE-19 cells after stimulation with LPS for 3 h are shown. The data are expressed as means ± standard errors of four independent experiments. *P < 0.01; NS not significant, compared to the value of LPS-alone-treated group