Fig. 6.

Effects of berberine on MCP-1 and IL-8 protein concentrations after stimulation with LPS in ARPE-19 cells were incubated for 30 min in serum-free media with berberine or 0.05% DMSO. LPS (1 μg/ml) was added to the medium and the cells were incubated for 24 h. The MCP-1 levels (a) and IL-8 protein concentrations (b) after stimulation with LPS in the culture media, determined using ELISA, are shown. The data are expressed as means ± standard errors of four independent experiments. *P < 0.01; NS not significant, compared to the value of LPS-alone-treated group