Skip to main content
. 2017 Apr 5;22(4):581. doi: 10.3390/molecules22040581

Figure 2.

Figure 2

Schematic diagram of the pHellsgate-CAS RNAi vector. LB: left border; P nos: NOS promoter; T nos: NOS terminator; RB: right border. The CAS RNAi fragment was inserted into the XbaI sites and the XhoI sites of the pHellsgate2 by means of inverted repeats. After this step of the homologous recombination reaction, the hairpin structure (contains an antisense target sequence, the PDK intron and a sense target sequence) was inserted between the 35S promoter (P 35S) and OCS terminator (T ocs) of the pHellsgate2 vector, and fulfilled the function of gene silencing [15,16].