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. 2017 May 19;22(5):844. doi: 10.3390/molecules22050844

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE analysis of recombinant BglG167b. Lane M, molecular weight standard; lane 1, crude extract of BL21 (DE3) carrying pGEX-BglG167b without induction; lane 2, soluble fraction in crude extract of BL21 (DE3) carrying pGEX-BglG167b after induction; lane 3, GST-BglG167b enzyme fraction after purification with the GST-bind agarose resin; lane 4, purified recombinant BglG167b after cleavage by thrombin2.5. Characterization of recombinant BglG167b.