TLC analyses of time-course of ginsenoside bioconversion by BglG167b at an enzyme concentration of 1.0 mg/mL. (A) the transformation of Rb1; (B) the transformation of Rb2; (C) the transformation of Rc; (D) the transformation of Rd; (E) the transformation of gypenoside XVII; (F) the transformation of Rg3(S); (G) the transformation of F2; (H) the transformation of Re; and (I) the transformation of ginsenoside Rg1. Developing solvent: CHCl3–CH3OH–H2O (70:30:10, v/v, lower phase). Lane S, standards (Rb1, Rd, F2, C-K, PPT, PPD); Lane S1, standards (Rb1, Rd, Rg3(S), Rh2(S), PPD); Lane S2, standards (Rb2, C-Y, PPD); Lane S3, standards (Rc, C-Mc, PPD); Lane S4, standards (Rb1, GypXVII, GypLXXV, Rh2(S), PPD); Lane S5, standards (Rg3(S), Rh2(S), PPD); Lane S6, standards (F2, Rh2(S), PPD); Lane S7, ginsenoside standards (Re, Rg2(S), Rh1(S), PPT); Lane S8, ginsenoside standards (Rg1, Rh1(S), PPT); Lane 1, control; Lane 2, 5 min; Lane 3, 30 min; Lane 4, 3 h; Lane 5, 10 h; Lane 6, 24 h; Lane 7, 48 h.