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. 2018 Sep 18;9:1967. doi: 10.3389/fimmu.2018.01967

Figure 7.

Figure 7

Overexpression of FIBCD1 on the surface of A549 cells inhibits TLR agonist effects. A549 sham- and FIBCD1-transfected cells were seeded at a density of 250,000 cells in 0.5 mL of media per well of a 24-well tissue culture plate and serum-starved overnight prior to stimulation. The cells were stimulated with TLR1/2 (A), 5 (F), and 6/2 (G) agonists (0.67 μg/mL), TLR2 (B) agonist (6.7·107 cells/mL), TLR3a (C) and 3b (D) agonists (8.9 μg/mL), TLR4 (E) agonist (4.4 μg/mL), TLR7 (H) and 8 (I) agonists (1.8 μg/mL), and TLR9 (J) agonist (0.068 μg/mL) for 8 h and the concentration of secreted IL-8 was determined by sandwich ELISA as described in methods. Data are presented as mean ± SEM from three independent experiments. Duplicate cell cultures were used for each of the three independent experiments and ELISA measurements were performed in duplicates on each of these. Data were analyzed by two-way ANOVA, followed by Tukey's post-test, #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 relative to DPBS-treated cells. *p < 0.05, **p < 0.01, and ***p < 0.001 relative to A549 sham cells stimulated with the same stimulant.