Fig. 9.

Sp1 inhibitor Mithramycin A kills low ALDH2 expressing cancer cells. a Clonogenic survival analysis performed in H1299 and JHH4 cells after two subsequent 24 h treatments (total 48 h) with Mithramycin A (Sp1 inhibitor). b qRT-PCR analysis of XRCC1 and APE1 mRNA levels in JHH4 cells after treatment with 50 nM or 500 nM Mithramycin A for 24 h. c qRT-PCR analysis of XRCC1 and APE1 mRNA levels in H1299 cells after treatment with 50 nM or 500 nM Mithramycin A for 24 h. qRT-PCR reference genes are B2M and GAPDH. Data are expressed as mean ± SD from at least three independent experiments. *** p < 0.001