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Effect of ARFW on MAPK signaling. Cells were treated with ARFW or LPS (100 ng/mL) for 30 min and MAPK protein expression levels were determined by western blot analysis with specific antibodies (A). Cells were treated with ARFW or LPS (100 ng/mL) in the presence of MAPK inhibitors for 30 min and MAPK protein expression levels were determined, Cells were treated with 20 μM of the MAPK inhibitor SB203580 (p38 inhibitor), SP600125 (JNK inhibitor), and PD098059 (ERK inhibitor) (B,C).
