FIGURE 1.

STUbL subunits Slx5 and Slx8 alleviate toxicity of poly-Q expanded Htt. (A) WT, slx5Δ, and slx8Δ strains expressing Htt-25Q, Htt-103Q, or empty vector (EV) were grown to mid-logarithmic phase and 5 μl of 10-fold serial dilutions of each culture were spotted on SC-URA medium. Plates were incubated at 30°C for 3 days. (B) Yeast transformants in A and the indicated controls were grown overnight in 5 ml of SC-URA medium. Ten OD₆₀₀ readings of cultures were recorded every hour until the OD₆₀₀ reached ∼2.0. The average doubling times of four independent experiments were graphed with +/− standard error. EV (empty vector) (C) A shuffle strain, slx5Δ with SLX5/TRP plasmid (YOK 2990), was transformed with either Htt-25Q or Htt-103Q constructs. Transformants were patched in duplicate on selective medium (SC-TRP URA) and rich medium (YPD). Patches were then replica plated on SC-URA medium with 5FAA to counter-select against the TRP1 marked plasmid.