Table 1.

MicroRNAs enriched in LPS- or VitD3-EV with known functions in DC

Identifier	Enriched in	FDR	Function in DC	References
mmu-miR-155-5p	LPS-EV	4.04E−12	Master regulator in DC maturation	[62]
mmu-miR-708-3p	VitD3-EV	2.21E−09	Downregulated in mature/activated DC	[63]
mmu-miR-10a-5p	VitD3-EV	2.31E−07	Inhibits DC activation and Th1/Th17 cell immune responses	[64]
mmu-miR-146a-5p	LPS-EV	4.53E−06	Down regulates IL-12p70, IL-6, and TNF-α production by DC	[65]
mmu-miR-9-5p	LPS-EV	1.86E−05	Regulatory circuitry controlling monocyte activation by LPS	[66]
mmu-miR-223-5p	VitD3-EV	6.82E−05	Repression of pro-inflammatory cytokine release by DC	[67]
mmu-miR-378a-3p	VitD3-EV	8.24E−05	Upregulated in VitD3-treated DC	[68]
mmu-miR-203-3p	VitD3-EV	0.000328	Upregulated in tolerogenic DC	[69]
mmu-miR-199a-3p	VitD3-EV	0.000484	Upregulated in tolerogenic DC	[69]
mmu-miR-27b-5p	VitD3-EV	0.000578	Suppression of inflammatory cytokine production via NF-κB	[69]
mmu-miR-7a-5p	LPS-EV	0.000703	Upregulated in LPS/IFNg stimulated DC	[69]
mmu-miR-126a-3p	VitD3-EV	0.000946	Reduces the responsiveness of DCs to TLR7/9 ligands	[70]
mmu-miR-708-5p	VitD3-EV	0.000946	Suppresses NF-κB signaling	[71]
mmu-miR-181b-3p	VitD3-EV	0.001923	Inhibition of CD40 and MHCII expression	[72]
mmu-miR-27a-5p	VitD3-EV	0.002511	Suppression of inflammatory cytokine production	[73]

EV-associated RNA from control, LPS, and VitD3 conditions was isolated and analyzed by RNA sequencing. Read counts for individual RNAs were normalized to the total read counts of each RNA class. LPS- or VitD3-induced fold-changes and corresponding p values were calculated relative to the control condition with edgeR GLM method. We created a top 20 list of miRNAs with the lowest FDR values. Indicated are miRNAs from this top 20 for which DC-related functions have been reported