Fig. 2.

Detection of autophagy. HT22 cells were treated with 1, 2, or 4 mM 3-FMC for 24 h. The relative protein levels of LC3-I, LC3-II, and p62 normalized to loading control GAPDH were quantitated by densitometry as described in Materials and Methods. Similar results were obtained in three independent experiments. C—control, untreated cells