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Conscious Balb/c mice underwent DRS for 5 minutes. Then, mice were anesthetically induced for 1 or 4 minutes with 4.0% or 1.5% isoflurane (Iso), respectively, and 1 L/min O2. Following induction, mice were transferred to a nose cone and underwent DRS for 15 minutes at 1.5% isoflurane and 1 L/min O2. Finally, mice were removed from the nose cone and the left flank underwent DRS for 5 minutes.
