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DRS was performed on Balb/c mouse (10 weeks old) to quantify physiological parameters (THC, StO2, HbO2, and μs’) in subcutaneous CT26 tumor allografts at a volume of 100 mm3 (b, e), 500 mm3 (c, f), and adjacent normal tissue (a, d) both with (d, e, f) and without isoflurane anesthesia (a, b, c). Anesthesia was constant at 1.5% isoflurane and 1 L/min O2.
