Fig. 2.

Effect of tadalafil on the threshold for the first myoclonic twitch (a), generalized clonus (b), and forelimb tonus (c) in the i.v. PTZ seizure threshold test in mice. Tadalafil and valproate (positive control) were administered i.p. 90 and 15 min before the test, respectively. The doses are shown on the abscissa. Control animals received 1% Tween 80. Each experimental group consisted of 10–13 animals. Each bar represents the mean (mg/kg PTZ) + SEM. *p < 0.05, ***p < 0.001 versus the control group (one-way ANOVA followed by the Tukey’s post hoc test)