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. 2018 Jul 5;165(2):291–301. doi: 10.1093/toxsci/kfy162

Figure 2.

Figure 2.

The serum antibody levels in NOD female (A–D) and male offspring (E–H). (A, E) IgG2a, (B, F) IgG2b, (C, G) IgM, and (D, H) IgG1 levels in the sera of NOD female and male offspring were measured at dilutions of 1:50, 1:50, 1:500, and 1:5000 (v/v), respectively, following titration by serial dilution using ELISA. The sera were obtained when the mice were euthanized. *, p < .05 and **, p < .01. Each of the serum antibody concentration was calculated as the percent control. VHF, females exposed to vehicle control (N = 21); GEF, females exposed to GEN (N = 16); VHM, males exposed to vehicle control (N = 10); GEM, males exposed to GEN (N = 16). GEN, genistein; ELISA, enzyme-linked immunosorbent assay.