Figure 8.
Hepatocyte-specific loss of TIPARP increases dioxin-dependent regulation of hepatic AHR target gene expression. Hepatic mRNA levels of Cyp1a1 (A), Cyp1a2 (B), Cyp1b1 (C), Tiparp (D), Ahrr (E), Nqo1 (F) Nfe2l2 (G) Serpine 1 (H) were determined after 3 days of exposure to CO or 10 μg/kg dioxin as described in experimental procedures (n = 4). Recruitment of AHR to Cyp1a1 (I) and Cyp1b1 (J). Data represent the mean ± SEM. Representative AHR (K), and β-actin protein levels were detected by Western blotting after 3 days of treatment. AHR proteins levels were normalized to β-actin levels, n = 4) (L). For all data, p < .05 was determined by 2-way ANOVA followed by Tukey’s post hoc test comparison. Significantly different compared with genotype-matched *DMSO- or #dioxin-treated Tiparpfl/fl mice.