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. 2018 Sep 25;5:180184. doi: 10.1038/sdata.2018.184

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Copyright © 2018, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

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(a) Primers design for the deletion construct. The forward primer (FP) of the DS region carries RS-A (restriction site-A) identical to the RS of the reverse primer (RP) of the US region found in the p2NIL vector. RS-B and RS-C are found on either sides of RS-A. (b) Design of the metabolites extraction method. The supernatant of cultures after centrifugation is used for the quantitation of extracellular metabolites. The extract from the cells pellet is used for the quantitation of intracellular metabolites. Derivatization was performed either during or post extraction depending on the method.