Table 1. Gene annotations and mutants used in this study.

Enzyme	EgtA	EgtB	EgtC	EgtD	EgtE	MshA
	Gamma-glutamylcysteine synthase	sulfoxide synthase	glutamine amidohydrolase	S-adenosylmethionine-dependent methyltransefrase	C-S lyase	Mycothiol glycosyl transferase
Gene	egtA	egtB	egtC	egtD	egtE	mshA
ORFa in M. smegmatis	MSMEG_6250	MSMEG_6249	MSMEG_6248	MSMEG_6247	MSMEG_6246	MSMEG_0924
ORF in M.tb (H37Rv)	Rv3704c	Rv3703c	Rv3702c	Rv3701c	Rv3700c	Rv0486
ORF in M.tb (CDC1551)	MT3807	MT3806	MT3805	MT3804	MT3803	MT0504
M. smegmatis mutants	_	_	_	Unmarked HRb mutant, ∆egtD12	_	Kanamycin marked, transposon mutant ∆mshA2
M.tb mutants (H37Rv)	_	Unmarked HR mutant, ∆egtB8	_	_	Unmarked HR mutant, ∆egtE8
M.tb mutants (CDC1551)	Unmarked HR mutant ∆egtA8	Unmarked HR mutant ∆egtB8	Unmarked HR mutant ∆egtC8	Unmarked HR mutant ∆egtD8	Unmarked HR mutant ∆egtE8	Hygromycin marked HR mutant, ∆mshA8

^aOpen reading frame.

^bHomologous recombination.