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. 2018 Sep 16;24:6506–6516. doi: 10.12659/MSM.910969

Figure 3.

Figure 3

MicroRNA-7-5p (mir-7-5p) negatively regulated the expression poly (ADP-ribose) polymerase 1 (PARP-1), which was involved in the regulation of apoptosis in cisplatin-resistant cervical cancer cells. (A) The target sites of microRNA-7-5p (mir-7-5p) in 3′UTR of poly (ADP-ribose) polymerase 1 (PARP-1) mRNA predicted by ReRNA2.0. (B) Luciferase reporter assay was conducted with the indicated reporter plasmid. (C, D) The protein levels of PARP-1 in cisplatin-resistant cervical cancer cells and control (parental) cells with indicated treatment were measured by Western blot. (E, F) The protein levels of PARP-1 in the control (parental) cells simultaneously transfected with miR-7-5p and pcDNA3/PARP-1 were measured by Western blot. (E) Apoptosis of the cisplatin-resistant cervical cancer cells, transfected with indicated plasmid, following cisplatin treatment was measured by flow cytometry. ** p<0.01, ns – no significance.