Figure 5.

MicroRNA-7-5p (mir-7-5p) promotes autophagy in cisplatin-resistant cervical cancer cells, which is essential for cell survival. (A) Control (parental) or cisplatin-resistant cervical cancer cells were transfected with the GFP/LC3 expression plasmid and then treated with cisplatin. Formation of autophagosomes were visualized with fluorescence microscopy. (B) Cisplatin-resistant cervical cancer cells were transfected with GFP/LC3 expression plasmid and ASO-miR-7-5p, and the formation of autophagosomes were visualized with fluorescence microscopy. (C) The ratio of ATP/ADP cisplatin-resistant cervical cancer cells with indicated treatments were measured. (D) Apoptosis of the cisplatin-resistant cervical cancer cells with indicated treatment was measured by flow cytometry. * p<0.05.