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. 2018 Sep 16;24:6506–6516. doi: 10.12659/MSM.910969

Figure 6.

Figure 6

MicroRNA-7-5p (mir-7-5p) modulates autophagy in cisplatin-resistant cervical cancer cells via regulating the expression of BLC2, which in combination with PARP-1, modulates the energy homeostasis. (A) The target sites of miR-7-5p in 3′UTR of BCL2 mRNA predicted by ReRNA2.0. (B) Luciferase reporter assay was conducted with the indicated reporter plasmid. (C) The protein levels of PARP-1 in cisplatin-resistant cervical cancer cells with indicated treatment were measured by Western blot. (D) Cisplatin-resistant cervical cancer cells were transfected with the GFP/LC3 expression plasmid simultaneously transfected with ASO-miR-7-5p and si-BCL2 and then treated with cisplatin. Formation of the autophagosomes were visualized with fluorescence microscopy. (E) The ratio of ATP/ADP in cisplatin-resistant cervical cancer cells with indicated treatment were measured. (F) Apoptosis of cisplatin-resistant cervical cancer cells transfected with the indicated plasmids under cisplatin treatment was measured by flow cytometry. (G) A diagrammatic summary of the findings of the study.