Table 2.
Calibration curves, LOD, LOQ, precision, repeatability, stability, and recovery of the 24 references.
| Compounds | Calibration Curves | R2 | Linear Range/μg/mL | LOD/ng/mL | LOQ/ng/mL | Precision (%, n = 6) | Repeatability (%, n = 6) | Stability (%, n = 6) | Recovery (%, n = 3) | |
|---|---|---|---|---|---|---|---|---|---|---|
| Mean | RSD | |||||||||
| 1. Danshensu | Y = 1449.8X − 477.58 | 0.9998 | 1.15~114.50 | 55.91 | 186.36 | 3.13 | 3.24 | 4.26 | 101.07 | 2.13 |
| 2.Protocatechuic aldehyde | Y = 23160X − 1692.6 | 0.9982 | 0.16~16.20 | 7.63 | 25.42 | 3.12 | 4.38 | 3.31 | 99.65 | 3.21 |
| 3. Caffeic acid | Y = 9923.3X − 221.72 | 0.9981 | 0.19~19.00 | 14.55 | 48.50 | 3.33 | 3.72 | 3.36 | 99.86 | 2.21 |
| 4. Rutin | Y = 8337.1X + 17508 | 0.9986 | 1.01~101.00 | 4.05 | 13.50 | 2.97 | 3.59 | 4.29 | 100.79 | 2.43 |
| 5. Isoquercitrin | Y = 6526.3X + 7495.6 | 0.9996 | 0.93~92.50 | 7.05 | 23.50 | 1.12 | 2.87 | 3.62 | 99.09 | 1.99 |
| 6. Astragalin | Y = 18160X − 3328.2 | 0.9994 | 0.74~74.00 | 4.09 | 13.64 | 2.11 | 4.39 | 2.42 | 101.91 | 3.21 |
| 7. Rosmarinic acid | Y = 2132.8X + 4654.1 | 0.9989 | 1.27~126.50 | 22.85 | 76.16 | 1.68 | 3.96 | 1.82 | 98.68 | 2.65 |
| 8. Lithospermic acid | Y = 375.37X + 1307.5 | 0.9915 | 1.90~380.00 | 93.37 | 311.24 | 2.10 | 4.10 | 3.64 | 99.76 | 3.21 |
| 9. Salvianolic acid B | Y = 1292.2X + 4951.3 | 0.9986 | 1.29~257.00 | 66.37 | 221.22 | 1.73 | 3.85 | 1.35 | 101.25 | 1.34 |
| 10. Salvianolic acid A | Y = 4008.9X + 172.38 | 0.9972 | 0.174~17.40 | 33.08 | 110.27 | 1.87 | 3.90 | 3.36 | 99.82 | 2.93 |
| 11. Tanshinone IIB | Y = 133524X + 35,802 | 0.9967 | 0.07~7.10 | 3.12 | 10.40 | 1.73 | 3.21 | 4.91 | 102.23 | 2.36 |
| 12.Dihydrotanshinone I | Y = 232666X + 126,802 | 0.9972 | 0.13~12.60 | 8.20 | 27.33 | 2.86 | 4.55 | 2.43 | 98.22 | 3.25 |
| 13.Cryptotanshinone | Y = 293851X + 578,254 | 0.9955 | 0.24~23.60 | 11.42 | 38.06 | 4.55 | 4.91 | 2.78 | 101.15 | 1.52 |
| 14.Neocryptotanshione | Y = 100.92X + 24.255 | 0.9976 | 0.19~18.60 | 213.67 | 712.29 | 2.86 | 3.15 | 3.12 | 100.18 | 2.83 |
| 15. Tanshinone I | Y = 32735X + 19,739 | 0.9961 | 0.11~10.70 | 40.04 | 133.48 | 2.25 | 3.48 | 3.47 | 99.82 | 3.29 |
| 16.Tanshinone IIA | Y = 355880X + 261,945 | 0.9942 | 0.113~11.30 | 5.24 | 17.47 | 4.74 | 1.19 | 4.26 | 102.34 | 1.58 |
| 17. Miltiradiene | Y = 794169X+ 561,247 | 0.9954 | 0.09~8.90 | 2.73 | 9.10 | 4.60 | 4.06 | 4.28 | 100.34 | 3.28 |
| 18. Miltirone | Y = 88227X + 109,759 | 0.9991 | 0.72~72.00 | 4.53 | 15.11 | 1.14 | 3.94 | 4.08 | 101.64 | 2.43 |
| 19. Oleanolic acid | Y = 8484.5X + 6510.4 | 0.9988 | 0.18~17.60 | 14.93 | 49.78 | 3.01 | 4.02 | 3.73 | 99.01 | 1.54 |
| 20. Ursolic acid | Y = 1064.6X + 5087.3 | 0.9979 | 1.04~103.50 | 38.37 | 129.91 | 2.57 | 4.44 | 4.15 | 99.90 | 3.24 |
| 21. Fructose | Y = 1.4383X + 5.879 | 0.9923 | 32~3200 | 4.14 × 104 | 1.38 × 105 | 1.34 | 2.78 | 3.11 | 100.35 | 2.45 |
| 22. Glucose | Y = 1.3669X + 6.6307 | 0.9937 | 30.54~3054.00 | 2.52 × 104 | 8.39 × 104 | 1.83 | 2.69 | 2.13 | 101.35 | 2.52 |
| 23. Sucrose | Y = 1.2227X + 7.9658 | 0.9941 | 26.06~2606.00 | 3.83 × 104 | 1.28 × 105 | 1.75 | 3.88 | 2.47 | 102.23 | 3.61 |
| 24. Stachyose | Y = 1.2012X + 7.9475 | 0.9900 | 50.26~5026.00 | 2.19 × 104 | 7.31 × 104 | 1.92 | 2.31 | 3.23 | 99.43 | 1.35 |