Table 1.
Results of the central composite design for the laccase production.
| Runs | Factors | Laccase Activity (U/mL) | |||
|---|---|---|---|---|---|
| X1 (Time, d) | X2 (Temperature, °C) | X3 (pH) | Exp.a | Pred.b | |
| 1 | −1 (2) | −1 (28) | −1 (5) | 5.63 | 5.46 |
| 2 | 1 (6) | −1 (28) | −1 (5) | 11.95 | 11.88 |
| 3 | −1 (2) | 1 (32) | −1 (5) | 4.01 | 3.84 |
| 4 | 1 (6) | 1 (32) | −1 (5) | 8.03 | 7.86 |
| 5 | −1 (2) | −1 (28) | 1 (7) | 6.44 | 6.30 |
| 6 | 1 (6) | −1 (28) | 1 (7) | 11.51 | 11.36 |
| 7 | −1 (2) | 1 (32) | 1 (7) | 7.59 | 7.32 |
| 8 | 1 (6) | 1 (32) | 1 (7) | 10.12 | 9.98 |
| 9 | −1.68 (0.64) | 0 (30) | 0 (6) | 1.82 | 2.12 |
| 10 | 1.68 (7.36) | 0 (30) | 0 (6) | 9.55 | 9.75 |
| 11 | 0 (4) | −1.68 (26.64) | 0 (6) | 11.68 | 11.88 |
| 12 | 0 (4) | 1.68 (33.16) | 0 (6) | 9.05 | 9.36 |
| 13 | 0 (4) | 0 (30) | −1.68 (4.32) | 7.43 | 7.66 |
| 14 | 0 (4) | 0 (30) | 1.68 (7.68) | 9.84 | 10.14 |
| 15 | 0 (4) | 0 (30) | 0 (6) | 16.28 | 16.24 |
| 16 | 0 (4) | 0 (30) | 0 (6) | 16.53 | 16.24 |
| 17 | 0 (4) | 0 (30) | 0 (6) | 16.49 | 16.24 |
| 18 | 0 (4) | 0 (30) | 0 (6) | 15.82 | 16.24 |
| 19 | 0 (4) | 0 (30) | 0 (6) | 15.97 | 16.24 |
| 20 | 0 (4) | 0 (30) | 0 (6) | 16.41 | 16.24 |
a Exp. is expressed as experimental values. b Pred. is expressed as predicted values.