Fig. 6.

In vivo analysis revealed that CH₄-mediated S-nitrosylation was sensitive to PTIO. Mung bean seeds were presoaked with solutions containing 1.3 mM CH₄, 100 μM SNP, 500 μM PTIO, alone or their combinations for 12 h, and then shifted to 20% PEG-6000 for another 48 h. Biotin labeled proteins were detected by Western blot with anti-biotin antibodies (a). Meanwhile, the equal protein amounts were evaluated by Coomassie staining (b) after SDS-PAGE separation. Numbers on the left of the panels indicate the position of the protein markers in kDa. The gels are representative of at lest five replicates per experiment