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. 2018 Jul 26;6(4):429–449. doi: 10.1016/j.jcmgh.2018.07.003

Figure 6.

Figure 6

Rarb relays the signal of glycemic variability to Runx3/Col6a1 via histone modification. (A) Sketch view of chromatin immunoprecipitation (ChIP) assay. (B) ChIP analysis of Rarb gene shows percentage input of H3K9me2, H2AK119ub, H3K27me3, H3K4me3, and H3K27ac in hyperglycemic and hypoglycemic PDAC cells. Data are presented as mean ± standard deviation, and data from 3 independent experiments are shown; H, hyperglycemic cells; L, hypoglycemic cells. (C) Quantitative RT-PCR analysis shows expression of Rarb, Runx3, and Col6a1 after 10 μmol/L, 20 μmol/L SAHA treatment for 24 hours. Data are presented as mean ± standard deviation, and data from 3 independent experiments are shown. Unpaired t test is applied, *P < .05. (D) ChIP analysis of Rarb gene shows percentage input of H3K9me2, H3K27me3, and H3K27ac in control and SAHA treated hypoglyemic (L) PDAC cells. Data are presented as mean ± standard deviation, and data of 2 independent experiments are shown.