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. 2018 Jul 26;6(4):429–449. doi: 10.1016/j.jcmgh.2018.07.003

Figure 7.

Figure 7

Rarb relays the signal of glycemic variability to Runx3/Col6a1 via promoter methylation. (A) Methylation-specific PCR analysis shows the methylation status of Rarb gene in hypoglycemic PDAC cells, but not in hyperglycemic PDAC cells; M.SssI, CpG methyltransferase. (B) Quantitative RT-PCR analysis shows the expression of Rarb, Runx3, Col6a1, and Egr2 after decitabine treatment in hypoglycemic PDAC cells. All data are presented as mean ± standard deviation, and data from 3 independent experiments are shown. Unpaired t test is applied, *P < .05. (C) Western blot analysis shows expression of Runx3 and Col6a1 in hypoglycemic 399 (left panel) and 634 (right panel) cells after restoration of hyperglycemic culturing conditions.