Skip to main content
. 2018 Sep 11;28(9):1153–1161. doi: 10.1089/thy.2017.0528

FIG. 3.

FIG. 3.

Tpo-R1aRap1BKO mice have a lower incidence rate of thyroid carcinoma due to increases in apoptosis relative to Tpo-R1aRap1A mice. (A and B) 20 × images of hematoxylin and eosin–stained thyroid sections from Tpo-R1aRap1A (A) and Tpo-R1aRap1B (B) mice. (C) Percent incidence of thyroid carcinoma in Tpo-R1aKO (n = 11 at nine months of age), Tpo-R1aRap1KO (n = 18 at nine months of age), Rap isoform-specific Tpo-R1aRap1AKO (n = 15 at six to nine months of age), and Tpo-R1aRap1BKO (n = 18 at nine months of age) mice. *p ≤ 0.05 compared to Tpo-R1aKO; #p ≤ 0.05 compared to Tpo-R1aRap1AKO. (D) Quantification of cleaved caspase-3 staining represented as a percentage of the total area of the image (n = 8 mice per genotype). **p ≤ 0.001 relative to Tpo-R1aRap1AKO. (E and F) Diaminobenzidine immunohistochemistry for cleaved caspase-3 (black arrows) on thyroid tissue sections from Tpo-R1aRap1AKO (E) and Tpo-R1aRap1BKO (F) at 40 × magnification.