Fig. 1.

The accuracy of RT- PCR was further validated by gel electrophoresis. RT- PCR products were run in 2% agarose gel and bands were seen at the desired size (ABCB1: 151 bp; ALDH1A1: 98 bp; OCT4: 145 bp; NANOG: 149 bp; and β2M: 69 bp). The negative control lanes are indicated by RT minus (no reverse transcriptase for the reverse transcription reaction) and NTC (no-template control for the PCR reaction). A molecular weight marker (50 bp ladder) is used