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. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Anal Chem. 2018 May 11;90(11):6835–6842. doi: 10.1021/acs.analchem.8b00986

Figure 3.

Figure 3.

DNAJB4 is down-regulated in metastatic melanoma cells. (a) Western blot for the validation of the expression levels of DNAJB4 in the three pairs of melanoma cells. 115, 266–4, 39, 37, 793, and 1205 denote WM-115, WM-266–4, IGR-39, IGR-37, WM-793, and 1205Lu cells, respectively. (b) PRM traces for the quantification of DNAJB4 protein in three pairs of melanoma cells from forward and reverse SILAC labeling experiments. (c) Quantitative comparison of ratios of DNAJB4 obtained from PRM and Western blot analysis (n = 3). (d) Kaplan–Meier survival analysis showing that higher levels of expression of the DNAJB4 gene confers better prognosis of melanoma patients.