Fig. 2.

Inhibition of TLR4 down-regulates the LPS induced CCL2. Antibody-treated cells were stimulated with LPS and incubated for 24 h. Cells and culture media were collected. Real time PCR data showed that neutralization of TLR4 significantly suppress LPS induction of CCL2 (A) and reduced CCL2 protein was determined in the culture media by ELISA (B). TLR4 siRNA transfected THP-1 cells showed reduced expression of TLR4 mRNA compared to the cells transfected with control siRNA (C). LPS induced CCL2 expression was significantly inhibited in TLR4 deficient cells at both mRNA (D) and protein (E) levels