Fig. 3.

MyD88 deficiency reduced the LPS induced production of CCL2. THP-1 XBlue defMyD cells (cells deficient in MyD88 activity) were treated with LPS (10 ng/ml) or PBS (Vehicle; control) or TNF-α (10 ng/ml; MyD88 independent stimulus). Cells and culture media were collected after 24 h. Real time PCR and ELISA data showed that LPS failed to induce CCL2 at both mRNA (A) and secreted protein (B) levels. XBlue defMyD cells derived macrophages and were treated with LPS (10 ng/ml) or TNF-α (10 ng/ml). CCL2 gene expression was not induced by LPS in MyD88 deficient cells (C and D)