Table 1.
Accurate masses of the deprotonated molecular ion ([M − H]−) and product ions and the retention times of verproside and its metabolites identified in human hepatocytes.
| Code | Compound Name | Retention Time (min) | Detected ([M − H]−) (m/z) | Error (ppm) | Product Ions (m/z) |
|---|---|---|---|---|---|
| Verproside | 7.25 | 497.12939 | −1.5 | 355.0772, 221.0450, 153.0184 | |
| M1 | Verproside glucuronide | 2.99 | 673.16138 | −0.9 | 497.1301, 335.0773, 221.0450, 175.0241 |
| M2 | Verproside glucuronide | 6.11 | 673.16138 | −0.9 | 497.1301, 335.0773, 221.0450, 175.0241 |
| M3 | Verproside sulfate | 7.36 | 577.08594 | −1.6 | 497.1301, 335.0773, 221.0451, 153.0184 |
| M4 | Picroside II | 11.70 | 511.14484 | −1.7 | 349.0928, 235.0608, 167.0341 |
| M5 | Isovanilloylcatalpol | 12.32 | 511.14484 | −1.7 | 349.0928, 235.0608, 167.0341 |
| M6 | Picroside II glucuronide | 4.87 | 687.17657 | −1.7 | 511.1458, 349.0930, 235.0609, 167.0342 |
| M7 | Isovanilloylcatalpol glucuronide | 7.98 | 687.17657 | −1.7 | 511.1458, 349.0930, 235.0609, 167.0342 |
| M8 | Picroside II sulfate | 8.16 | 591.10150 | −1.5 | 511.1457, 349.0927, 235.0609, 167.0341 |
| M9 | Isovanilloylcatalpol sulfate | 9.44 | 591.10150 | −1.5 | 511.1457, 349.0927, 235.0609, 167.0341 |