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. 2017 May 15;22(5):806. doi: 10.3390/molecules22050806

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of bioreactors on the production of serglycin, heparin/heparan sulfate and chondroitin sulfate. The schematic indicates the structure of serglycin with eight glycosaminoglycan attachment sites that can be decorated with either chondroitin/dermatan sulfate or heparin/heparan sulfate chains. The effect of glycosaminoglycan lyase digestion on the glycosaminoglycan chains are indicated in panels (B,C) with HepIII removing heparin/heparan sulfate chains to reveal a single stub structure and chondroitinase ABC (C’ase ABC) removing chondroitin/dermatan sulfate chains to reveal a stub structure. ELISA for the presence of (A) serglycin core protein; (B) heparin/heparan sulfate stubs detected using anti-heparan sulfate/heparin-stub antibody clone 3G10 following HepIII digestion, and (C) chondroitin sulfate stubs detected using anti-4-sulfated chondroitin sulfate stub antibody clone 2B6 and anti-6-sulfated chondroitin sulfate stub antibody clone 3B3 following C’ase ABC digestion. Data are presented as means ± standard deviation (n = 3). * indicates significant differences (p < 0.05) compared to tissue culture flasks analyzed by one-way ANOVA.