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Synthesis of 4-thio-2’-deoxyuridine triphosphate (3) and N4-amino-2’-deoxycytidine triphosphate (4). Reagents and conditions: (i) Ac2O, NaOAc, 90 °C, 30 min; (ii) Lawesson’s reagent, toluene, 90 °C, 2 h; (iii) NaOCH3, CH3OH, rt, 30 min; (iv) POCl3, Bu3N, trimethyl phosphate, 0 °C, 90 min; then Bu3N, (NHBu3)2H2P2O7, CH3CN, 0 °C, 15 min; (v) NH2-NH2, H2O, rt, 18 h.
