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Effect of SAC on the increase of myofibroblasts in the lung. Double immunofluorescence staining for desmin (red) and α-smooth muscle actin (α-SMA, green) in lung was visualized using confocal microscopy. Representative microscopic scans are shown of (A) vehicle + saline; (B) SAC + saline; (C) vehicle + bleomycin, and (D) SAC + bleomycin groups. Arrowheads indicate immunopositive myofibroblasts, and V indicates vascular structure (magnification 200×). Scale bar = 50 μm.
