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Permeability of L. (L.) infantum plasma membrane incubated with compound 1 assessed spectrofluorimetrically by the fluorescence of the SYTOX Green. Promastigotes were treated with 1 (3 and 6 μM) for 60 min. At the end of this treatment, Triton X-100 was used for 100% permeabilization. Dimethyl sulfoxide (DMSO) was used as internal control and did not cause any alteration in membrane permeability (data not shown).
