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. 2017 Apr 29;22(5):715. doi: 10.3390/molecules22050715

Figure 7.

Figure 7

Effect of MBPF on LPS-induced activation of the nuclear factor κB (NF-κB) pathway in RAW264.7 cells. Cells were pretreated with various concentrations of MBPF (0, 11, 33, and 100 μM) for 2 h and were then stimulated with or without LPS (1 μg/mL) for 30 min. The protein levels of phospho-p65, phospho-IκB-α, and IκB-α were analyzed using Western blot (A–D). Cytosolic (C) and nuclear (N) protein were extracted to determine the levels of p65 using Western blot (E–H). The data represent the means ± S.E. of three independent experiments. (### p < 0.001 vs. control group; ** p < 0.01, *** p < 0.001 vs. LPS group).