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. 2018 Sep 14;9(72):33634–33647. doi: 10.18632/oncotarget.25950

Figure 2. Validation of LIF and LIFR expression data from OLINK screen and investigation of expression and secretion profile of LIF and LIFR at 0 Gy and 24 hours post 2 Gy irradiation.

Figure 2

(A) Secreted LIF protein from OE33P and OE33R cells which have been-mock irradiated and following 2 Gy X-ray irradiation was evaluated by ELISA, (n=4). (B) Relative expression of LIF mRNA in OE33P and OE33R cells at baseline and following 2 Gy Irradiation was evaluated by qPCR, (n=5). (C) Relative expression of LIFR mRNA at baseline in OE33R and OE33P cells and post 2 Gy irradiation was evaluated by qPCR, (n=5). Unpaired t-test used to compare OE33P and OE33R cell lines, Paired t-test used to compare within same cell lines. *p<0.05, **p<0.01.