Effect of IL-12p35 KO on DOX-induced endoplasmic stress and autophagy. (A). Representative images and quantification of p-PERK, p-eIF2a, ATF4, CHOP, Cle-cas12 and GAPDH by western blotting in the control, p35 KO, DOX and DOX + p35 KO groups. (B). Representative images and quantification of beclin 1, LC3–1/II and GAPDH by western blotting in each group. (C). Representative transmission electron micrographs indicating the formation of an autophagosome (5000×); N = 6 in each group. *P < 0.05 vs. the control group, and #P < 0.05 vs. the DOX group.