Fig. 9.

Effect of rIL-12 and rIL-35 on DOX-induced cardiac injury. (A). Heart sections were obtained from the three indicated groups and stained with HE (400×). The number of vacuolated cardiomyocytes was quantified; N = 5 in each group. Heart LDH (B) and CK-MB (C) levels were assessed; N = 6 in each group. (D). Level of STAT3 phosphorylation in each group was measured; N = 5 in each group. The levels of iNOS and Arg-1 (E); Nox2, Nox4, Nrf2 and HO-1 (F); Bax, Bcl2, Cyto C, Cle-cas9 and Cle-cas3 (G); p-PERK, p-eIF2a, ATF4, CHOP and Cle-cas12 (H); and beclin 1 and LC3-I/II were detected by western blotting in each group; N = 5 in each group. *P < 0.05 vs. the DOX + PBS group.