Figure 4.

HDAC4 gene silencing caused apoptosis of DLBCL cells and increased expression of BCLAF1.(A) Relative HDAC4 mRNA expression in OCI-LY10 cells treated with or without si-NC and si-HDAC4.(B) The protein levels of HDAC4, BCLAF1, p-IκB-α and p-p65 were assessed by Western blot. (C)The protein levels of p-IκB-α and p-p65 were assessed by Western blot. (D)Cell apoptosis rates were tested by flow cytometry. The expression of BCLAF1 at the mRNA level was also analyzed by q-RT-PCR after si-HDAC4 and Bay11-7082 treatment. All experiments were performed three times.Data are represented as mean±SD.*P<0.05,**P<0.01 versus the control group.