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. 2018 Aug 1;19(9):835–846. doi: 10.1080/15384047.2018.1480281

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Figure 5. — Silencing of Sirt2 enhanced sensitivity to DCA. (A) Cells were incubated with the indicated concentrations of drugs for 72h and cell survival was detected by CCK8 assay. Each bar represents the means ± SD of three separate experiments. (B) DCA-induced cell growth inhibition after siRNA transfection was measured by CCK8 assay. (C) DCA-induced ROS generation after siRNA transfection was measured by flow cytometry. (D) Cell were transfected with siRNAs against SIRT2 and whole cell lysates were subjected to immunoblotting for detection of PDHA1 and p-PDHA1 (S293) 72hr later. (E) Whole cell lysates from the indicated stable cell lines with AGK2 treatment for 12hor 48h after Sirt2 overexpression were IPed with anti-Flag gel, and subsequently immunoblotted with an anti-panacetyllysine and Flag antibody.