Figure 5.

Knock down of ATM or AMPK prevents the dephosphorylation of ULK-1 S757, mTOR S2448 and mTOR S2481, and the phosphorylation of ULK-1 S317. A. and B. Spiky ovarian cancer cells and BT474 mammary cancer cells were treated with vehicle control or with niraparib (2.0 μM), for 6h. Cells were fixed in place and at least forty cells per condition were imaged in independent triplicate and the intensity ratio of phosphorylated protein levels to total protein expression plotted as a percentage of control treatment (n = 3 +/- SD). # p < 0.05 greater than vehicle control; * p < 0.05 less than vehicle control.