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Identification of CTCs and CTC clusters from NB patients.
a1, b1) Nuclei were stained with DAPI (blue fluorescence). a2, b2) Chromatin was stained with CEP8 (orange fluorescence). a3, b3) White blood cells (WBC) were stained with antibody to CD45 (red circle around the cell surface). a4) Identification of a CTC (DAPI+/CD45-/CEP8 ≥ 3 pots), and identification of WBCs (DAPI+/CD45+/CEP8 = 2 spots). b4) CTC cluster, defined as more than two CTCs adhered together. (Scale bar = 10 μm).
