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. 2018 Aug 10;35:334–344. doi: 10.1016/j.ebiom.2018.07.042

Fig. 3.

Fig. 3

CREB5 DMR regulates gene expression through recruiting P53 and SP1. (a) The pGL3-basic vector with methylated or unmethylated CREB5 DMR were co-transfected with pGL3-SV40 vector (negative control) in three different cell lines, HTR8-S/Vneo cell, JEG-3 cell and 293 T cell. Firefly/Renilla ratio was normalized to the empty vector. (b) ChIP-qPCR showing P53 enrichment over CREB5 DMR in HTR8-S/Vneo cells. (c) ChIP-qPCR showing SP1 enrichment over CREB5 DMR in HTR8-S/Vneo cells. **P < 0·01, ***P < 0·001. All histograms represent mean ± SEM.